Coding

Part:BBa_K2680532:Design

Designed by: Julia Urban   Group: iGEM18_William_and_Mary   (2018-10-13)


sfGFP with BsaI sites removed


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


Design Notes

Sequence sent to William and Mary iGEM from Murray lab at the California Institute of Technology.


Source

sfGFP is an engineered GFP variant. See references.

References

Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C., & Waldo, G. S. (2006). Engineering and characterization of a superfolder green fluorescent protein. Nature Biotechnology, 24(1), 79–88. https://doi.org/10.1038/nbt1172